Spatiotemporal image correlation spectroscopy measurements of flow demonstrated in microfluidic channels

Spatiotemporal Image Analysis for Fluid Flow Measurements

In this chapter, a framework will be presented for measuring and modeling transport processes using novel visualization techniques and extended optical flow techniques for digital image sequence analysis. In this way, parameters besides the 2-D xy velocity components can be extracted concurrently from the acquired 2-D image sequences, such as wall shear rates and momentum transport close to bou...

New Insight from Using Spatiotemporal Image Correlation in Prenatal Screening of Fetal Conotruncal Defects

Surface-directed boundary flow in microfluidic channels.

Channel geometry combined with surface chemistry enables a stable liquid boundary flow to be attained along the surfaces of a 12 microm diameter hydrophilic glass fiber in a closed semi-elliptical channel. Surface free energies and triangular corners formed by PDMS/glass fiber or OTS/glass fiber surfaces are shown to be responsible for the experimentally observed wetting phenomena and formation...

Flow-induced deformation of shallow microfluidic channels.

We study the elastic deformation of poly(dimethylsiloxane) (PDMS) microchannels under imposed flow rates and the effect of this deformation on the laminar flow profile and pressure distribution within the channels. Deformation is demonstrated to be an important consideration in low aspect ratio (height to width) channels and the effect becomes increasingly pronounced for very shallow channels. ...

Scanning image correlation spectroscopy.

Molecular interactions are at the origin of life. How molecules get at different locations in the cell and how they locate their partners is a major and partially unresolved question in biology that is paramount to signaling. Spatio-temporal correlations of fluctuating fluorescently tagged molecules reveal how they move, interact, and bind in the different cellular compartments. Methods based o...